lentiviral vector encoding v5 tagged turboid (Addgene inc)

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Addgene inc lentiviral vector encoding v5 tagged turboid
Lentiviral Vector Encoding V5 Tagged Turboid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plx304+lentiviral+vector/C1(1-29)-TurboID-V5_pLX304+(Plasmid+%23107175)/pm41416371-302-2-14
Average 94 stars, based on 7 article reviews

lentiviral vector encoding v5 tagged turboid - by Bioz Stars, 2026-10

94/100 stars

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Article Title: Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway
Article Snippet: The detailed procedures were performed as reported previously ( , ) using 3 μg of ChIP-grade antibody against NRF2 (#ab62352, Abcam). .. Open reading frame (ORF) constructs for human GCLM , GCLC , PRDX1 , and GSR genes were purchased (Dharmacon, Lafayette, CO, USA; GCLM, #OHS6085-213573329; GCLC, #OHS6085-213573328; PRDX1, #OHS1770-202318473; GSR, #OHS1770-202312884) and cloned into the pLX304 lentiviral vector (Addgene plasmid 25890) using Gateway Technology (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA). .. For silencing of the indicated genes, two independent shRNAs were designed for each target and were cloned into the pLenti6/BLOCK-iT lentiviral vector (Invitrogen/Thermo Fisher Scientific).

Article Title: Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway.
Article Snippet: The detailed procedures were performed as reported previously (38, 42) using 3 g of ChIP-grade antibody against NRF2 (#ab62352, Abcam). .. Open reading frame (ORF) constructs for human GCLM, GCLC, PRDX1, and GSR genes were purchased (Dharmacon, Lafayette, CO, USA; GCLM, #OHS6085-213573329; GCLC, #OHS6085-213573328; PRDX1, #OHS1770-202318473; GSR, #OHS1770-202312884) and cloned into the pLX304 lentiviral vector (Addgene plasmid 25890) using Gateway Technology (Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA). .. For silencing of the indicated genes, two independent shRNAs were designed for each target and were cloned into the pLenti6/BLOCK-iT lentiviral vector (Invitrogen/Thermo Fisher Scientific).

Clone Assay:

Article Title: NAD + boosting reduces age-associated amyloidosis and restores mitochondrial homeostasis in muscle
Article Snippet: .. The pCAX APP Swe/Ind plasmid was purchased from Addgene (Plasmid #30145) and the human APP Swe/Ind gene was cloned into pLX304 lentiviral vector (Addgene, Plasmid #25890) by gateway cloning. .. The newly generated pLX304-APP Swe/Ind vector and lentiviral plasmid packaging mix (pCMV-dR8.2 with pCMV-VSV-G) were transfected into HEK293T cells by Lipofectamine 2000 (Thermo, #11668019).

Article Title: C5AR1-induced TLR1/2 pathway activation drives proliferation and metastasis in anaplastic thyroid cancer.
Article Snippet: Funding information Hebei Provincial Department of Science and Technology Health Innovation Project, Grant/Award Numbers: 22372409D, 20231063; Hebei Province Key Science and Technology Research Program, Grant/Award Number: 20240188; Natural Science Foundation of Hebei Province, Grant/Award Number: H2022206418 Abstract This study aimed to elucidate the role and mechanisms of Complement C5a receptor 1 (C5AR1) in driving the malignant progression of anaplastic thyroid carcinoma (ATC).. C5AR1 expression was assessed in ATC tissues and cell lines.. Functional assays evaluated the effects of C5AR1 knockdown on the malignant features of ATC cells.

Plasmid Preparation:

Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids
Article Snippet: .. Simultaneously, the pLX304 lentiviral vector (Addgene 25890) was digested with the same enzymes, and the large vector backbone was purified. ..

Article Title: Functional genomics using CRISPR-Cas systems, compositions, methods, screens and applications thereof
Article Snippet: .. Methods and Materials Cell lines and vectors: Materials were obtained from the following sources: HL-60 were kindly provided from the Whitehead Institute, Cambridge, Mass., USA; pCW57.1 Dox-inducible lentiviral vector, pX330-U6-Chimeric_BB-CBh-hSpCas9 vector, pLX304 lentiviral vector, and gRNA_AAVS1-T2 vector from Addgene. .. Methods and Materials Cell lines and vectors: Materials were obtained from the following sources: HL-60 were kindly provided from the Whitehead Institute, Cambridge, Mass., USA; pCW57.1 Dox-inducible lentiviral vector, pX330-U6-Chimeric_BB-CBh-hSpCas9 vector, pLX304 lentiviral vector, and gRNA_AAVS1-T2 vector from Addgene.

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Article Title: The roles for branch shelters and sheep manure to accelerate the restoration of degraded grasslands in northern China
Article Snippet: The pLX304 lenti-mCherry-SEpHluorin plasmid was generated by inserting an mCherry-SEpHluorin fragment from an mCherrySEpHluorin plasmid (Addgene #32001) into lentiviral vector pLX304 (Addgene #25890).

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